The BiOLiX STV Line

Automated Bio-Libraries

-196°C to -130°C

Number of biosample collections requiring cryopreservation is rapidly growing together with the necessities of sample management. Interest in automation at cryo level temperatures (down to -196°C) is unprecedented. LICONIC, industry leader in automated sample handling solutions for 25 years, has responded with a truly capacity scalable, fully automated, vapor phase LN2 cryostorage solution.

The STV series was implemented in 2013 for a large scale population cohort study with daily sample input calculated in thousands. The technology is scalable down to applications of ‹50,000 samples. LICONIC once again applies the BiOLiX Bio-Libraries principle ‘built fit-for-purpose’ to provide an industry leading solution for a dynamically changing automated storage application requirement.

STV Line Overview

BiOLiX STV

Main Features

Vapor phase nitrogen (dry storage) for optimal sample safety
High density and energy efficient cryogenic vessels
Proven and reliable overhead trolley rack transport
Full rack or individual sample retrieval options
Simple and effective robotics
Fully featured system control software
LIMS integration

The STV Line is the first on the market automated cryogenic (‹-150°C) storage system with configurable capacity. STV systems employ LICONIC‘s unique high density, motorized, vapor phase LN2 cryogenic vessels. Samples are retrieved from storage in a rack format. Optional sample picking provides a fully automated retrieval process.

LICONIC offers automated cryogenic storage solution addressing data precision, personal safety and sample integrity issues – all the challenges present in a manual access cryogenic processes. The STV Line provides the benefits of automation together with the features of LiCONiC‘s unique cryogenic storage tanks. The STV is ideal for collections from 50 000 to over 30 000 000 samples.

Applications include storage of:
body fluids, cell lines, cord blood, stem cells, tissues, etc.

STV example cross section

Benefits

Proven long term sample integrity
Extremely high storage density
Samples not exposed to room temperature during retrieval
Efficient and safety sample storage and retrieval
Precise data management and sample tracking
Intuitive system operation
Seamless LIMS integration

Temperature Range and Capacities

LiCONiC‘s BiOLiX series is the market‘s most complete selection of products for automated biobanking.
Products are categorized by application, temperature and capacity.

BOLIX STV Line examples			STV1k8-CT		STV73k5-CT		STV337k9-CT
Labware	Plate height	Pitch	Levels	Samples	Levels	Samples	Levels	Samples
FluidX 0.3 ml Screw Cap	19.1 mm	25 mm	40	161‘280	60	6‘508‘800	60	29‘940‘480
Micronic 0.5 ml Screw Cap	28.5 mm	35 mm	28	112‘896	43	4‘664‘640	43	21‘457‘344
LVL LX1000	45.6 mm	52 mm	19	76‘608	29	3‘145‘920	29	14‘471‘232
Thermo Matrix 1.0ml Screw Cap	54.6 mm	61 mm	16	64‘512	24	2‘603‘520	24	11‘976‘192
Greiner Bio One 2 ml	48.0 mm	54 mm	18	36‘288	28	1‘518‘720	28	6‘986‘112

Storage Conditions

The safety of samples in the STV system is built around the proprietary storage tank and system design. Extremely efficient insulation design ensures a small temperature gradient from the bottom to the top of the tank and long temperature hold times in case of LN2 supply disruption. Storage tanks can be moved in and out of the system safely and efficiently.

The STV Line ensures the safety and viability precious cell stocks and tissue specimens by limiting transfer for any sample movements within the automation envelope. Robotic transfers between critical temperature zones (storage locations and the sample picking platform) are done very quickly through ultra-dry refrigerated climate. No sample goes directly from storage to room temperature as with other systems. Refrigeration to also keeps the robotic area free of frost.

Space Efficiency

The STV Line Automated Bio-Libraries make best use of given room constraints by its “built to space” modular design. System dimensions, layout are optimized to the individual application’s needs and workflow requirements.